Université Laval
Faculté des sciences
et de génie

Alain Garnier, ing.

Professeur titulaire

Alain Garnier collabore, avec des équipes pluridisciplinaires, au développement de thérapies cellulaires contre des maladies incurables et contribue également à la conception de nouvelles technologies biologiques vertes et durables.

Champs d'intérêts

  • Bioingénierie
  • Conception, développement, optimisation et mise à l’échelle de bioprocédés
  • Production de métabolites, protéines recombinantes, vecteurs viraux, vaccins et cellules thérapeutiques
  • Ingénierie des cultures cellulaires
  • Bioprocédés environnementaux, énergétiques, verts et durables

  • GCH-2100 Génie biochimique II
  • GCH-2105 Assainissement industriel
  • GCH-2005 Cinétique et catalyse
  • GCH-2006 Calcul des réacteurs chimiques
  • GCH-6000 Communication scientifique orale et écrite I
  • GCH-7003 Cinétique biochimique
  • GCH-8001 Communication scientifique orale et écrite II

  • V. K. Lazarjan, A. B. Gashti, M. Feshki, A. Garnier and B. Gosselin. (2021). "Miniature Fiber-Spectrophotometer for Real-Time Biomarkers Detection". IEEE Sensors J. 21(13): 14822-14837.
  • Rasouli H, Iliuta I, Bougie F, Garnier A, Iliuta MC. (2021). "Enzyme-immobilized flat-sheet membrane contactor for green carbon capture". Chem Eng J. 421(1): 129587
  • Vincent T, Gaillet B, Garnier A. (2021). "Optimization of operation conditions for improved cytochrome P450BM3 enzymatic reaction yield". Can J Chem Eng. Online preview
  • Vincent T, Gaillet B, Garnier A (2021), "Optimisation of Cytochrome P450 BM3 Assisted by Consensus-Guided Evolution". Appl Biochem Biotechnol, early access, DOI: 10.1007/s12010-021-03573-1.
  • Awad G, Garnier A (2021), "Maximization of saturated fatty acids through the production of P450BM3 monooxygenase in the engineered Escherichia coli". Food Bioprod Process, 126:130-142.
  • Awad G, Garnier A (2021), "Promising optimization of bacterial cytochrome P450BM3 enzyme production by engineered Escherichia coli BL21". Biocat Agric Biotechnol, 31:101917. DOI: 10.1016/j.bcab.2021.101917.
  • Mangion M, Gelinas JF, Gashti ABZ Azizi H, Kiesslich S, Nassoury N, Chahal P, Kobinger G, Gilbert R, Garnier A, Gaillet B, Kamen A (2020), "Evaluation of novel HIV vaccine candidates using recombinant vesicular stomatitis virus vector produced in serum-free Vero cell cultures". Vaccine, 38(50):7949-7955.
  • Azizi D, Larachi F, Garnier A, Lague P, Levasseur B (2020), "Sorption of Aqueous Amino Acid Species on Sulfidic Mineral Surfaces – DFT Study and Insights on Biosourced-Reagent Mineral Flotation". Can J Chem Eng, 99(8):1758-1779.
  • Bahrami A, Iliuta I, Garnier A, Larachi F, Vincent V, Iliuta MC (2019):"Kinetics of Enzymatic Hydroxylation by Free and MNPs-Immobilized NADH-Dependent Cytochrome P450 BM3 from Bacillus megaterium". Ind Eng Chem Res, 58(2):808-815.
  • Laberge A, Ayoub A, Arif S, Larochelle S, Garnier A, Moulin VJ (2019), "α-2-Macroglobulin induces the shedding of microvesicles from cutaneous wound myofibroblasts". J Cell Phys, 234(7):11369-11379.
  • Hoesli CA, Tremblay C, Juneau PM, Boulanger MD, Beland AV, Ling SD, Gaillet B, Duchesne C, Ruel J, Laroche G, Garnier A (2018), "Dynamics of Endothelial Cell Responses to Laminar Shear Stress on Surfaces Functionalized with Fibronectin-Derived Peptides". ACS Biomater Sci Eng, 4(11):3779-91.
  • Bahrami A, Garnier A, Larachi F, Iliuta, MC (2018), "Covalent immobilization of cytochrome P450 BM3 (R966D/W1046S) on glutaraldehyde activated SPIONs". Can J Chem Eng, 96: 2227-2235.
  • Ghio SC, Larouche D, Garnier A, Germain L (2018), “Development of a keratinocyte-specific defines media suitable for self-assembled bilayered skin substitute production”. Cytotherapy, 20(5):S85-S86.
  • Del Guidice T, Lepetit-Stoffaes JP, Bordeleau LJ, Roberge J, Theberge V, Lauvaux C, Barbeau X, Trottier J, Dave V, Roy DC, Gaillet B, Garnier A, Guay D (2018), “Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cells”. Plos One, 13(4). DOI: 10.1371/journal.pone.0195558
  • Bahrami, A, Vincent, T, Garnier, A, Larachi, F, Boukouvalas, J, Iliuta, M (2017), “Non-covalent immobilization of optimized bacterial cytochrome P450 BM3 on functionalized magnetic nanoparticles”. Ind Eng Chem Res, 56(39):10981-10989.
  • Juneau PM, Garnier A, Duchesne C (2017), "Monitoring of adherent live cells morphology using the Undecimated Wavelet Transform Multivariate Image Analysis (UWT-MIA)". Biotechnol Bioeng, 114(1):141-153.
  • Ouellet, DL, Rousseau, J, Cherif, K, Gerard, C, Gilbert, R, Garnier, A, Gaillet, B, Beauséjour, C, Tremblay, JP. (2016). "Deletion of Mutated GAA Repeats from the Intron 1 of the Frataxin Gene Using the CRISPR System Restores the Protein Expression in a Friedreich Ataxia Model. Molecular Therapy. 24: S129-S129.
  • Tremblay-Bouliane, K., Tremblay, D., Moineau, S., Gaillet, B., Garnier, A. (2016), "Screening peptide-ore interactions with a phage display library for bioflotation application". CIM J, 7 (3).
  • Parent, V.A., Tremblay, J.P., Garnier, A. (2016), "Rational design of a serum-free culture medium for the growth of human myoblasts destined to cell therapy". Can. J. Chem. Eng., 94(10):1855–1862.
  • Brassard JA, Fekete N, Garnier A, Hoesli, CA (2016), " Hutchinson-Gilford progeria syndrome as a model for vascular aging". Biogerontol, 17(1):129-145.
  • Iliuta I, Garnier A, Iliuta MC (2015), "Enzymatic Fatty Acid Hydroxylation in a Liquid–Liquid Slug Flow Microreactor". Ind Eng Chem Res, 54:7787-7799.
  • Garnier A, Gaillet B (2015), "Analytical Solution of Luedeking-Piret Equation for a Batch Fermentation Obeying Monod Growth Kinetics". Biotechnol Bioeng, 112(12):2468-2474.
  • Juneau PM, Garnier A, Duchesne C (2015), "The Undecimated Wavelet Transform-Multivariate Image Analysis (UWT-MIA) for simultaneous extraction of spectral and spatial information”. Chemom. Int Lab Syst, 142:304-318
  • Hoesli CA, Juneau PM, Chevallier P, Duchesne C, Garnier A, Laroche G (2014), "A fluorophore-tagged RGD peptide to control endothelial cell adhesion to micropatterned surfaces". Biomaterials, 35(3), 879-890.
  • Segura MM, Mangion M, Gaillet B, Garnier A (2013), "New developments in lentiviral vector design, production and purification". Expert Opin Biol Ther, 13(7):987-1011.
  • Juneau PM, Garnier A, Duchesne C (2013), "Selection and tuning of a fast and simple phase-contrast microscopy image segmentation algorithm for measuring myoblast growth kinetics in an automated manner". Microsc Microanal, 19(4), 855-866.
  • Michaud FT, Havugimana PC, Duchesne C, Sanschagrin F, Bernier A, Lévesque RC, Garnier A (2012), "Cell culture tracking by multivariate analysis of raw LCMS data". Appl Biochem Biotechnol, 167(3):474-488.
  • Leysi-Derilou Y, Duchesne C, Garnier A, Pineault N (2012), "Single Cell Level Analysis of Megakaryocyte Growth and Development". Differentiation, 83:200-209.
  • Robert A, Cortin V, Garnier A, Pineault N (2012), "Megakaryocyte and platelet production from human cord blood stem cells". Methods Mol Biol, 788:219-247.
  • Pineault N, Cortin V, Boyer L, Garnier A, Robert A, Thérien C, Roy DC (2010), "Individual and synergistic cytokine effects controlling the expansion of cord blood CD34+ cells and megakaryocyte progenitors in culture". Cytotherapy, 13(4):467-480.
  • Gaillet B, Gilbert R, Broussau S, Pilotte A, Malenfant F, Mullick A, Garnier A, Massie B (2010), "High-level recombinant protein production in CHO cells using lentiviral vectors and the cumate gene-switch". Biotechnol Bioeng, 106(2):203-215.
  • Leysi-Derilou Y, Robert A, Duchesne C, Garnier A, Boyer L, Pineault N (2010), « Polyploid megakaryocytes can complete cytokinesis ». Cell Cycle, 9(13):2587-2597.  
  • Caron AW, Nicolas C, Gaillet B, Ba I, Pinard M, Garnier A, Massie B, Gilbert R (2009), "Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins". BMC Biotechnol, 9:42-52.
  • Michaud, FT, Garnier, A, Lemieux, L, Duchesne, C (2008), "Multivariate analysis of single quadrupole LCMS spectra for routine characterization and quantification of intact proteins". Proteomics Proteomics, 9 (3):746-756.
  • Segura MM, Garnier A, Di Falco MR, Whissell G, Meneses-Acosta A, Arcand N, Kamen A (2007), " Identification of host proteins associated with  retroviral vector particles by proteomic analysis of highly purified vector preparations". J Virol, 82:1107-1117.
  • Segura MM, Garnier A, Durocher Y, Coelho H, Kamen A (2007), "Production of Lentiviral Vectors by Large-Scale Transient Transfection of Suspension Cultures and Affinity Chromatography Purification". Biotechnol Bioeng, 98 (4), 789-799.
  • Gilbert PA, Kamen A, Bernier A, Garnier A (2007), "A simple macroscopic model for the diffusion and adsorption kinetics of r-adenovirus". Biotechnol Bioeng., 98:239:251.
  • Gaillet B, Gilbert R, Amziani R, Guilbault C, Gadoury C, Mullick A, Garnier A, Massie, B (2007), "High-level recombinant protein production in CHO cells using an adenoviral vector and the cumate gene-switch". Biotechnol Prog, 23(1):200-209.
  • Paradis-Bleau C, Cloutier I, Lemieux L, Sanschagrin F, Laroche J, Auger M, Garnier A, Levesque R (2007), "Peptidoglycan lytic activity of the Pseudomonas aeruginosa phage PhiKZ gp144 lytic transglycosylase". FEMS Microbiol Lett, 266:201-209.
  • Segura MM, Kamen A, Lavoie M-C, Garnier A (2007), "Exploiting heparin-binding properties of MoMLV-based retroviral vectors for affinity chromatography". J Chrom B, 846:124-131.
  • Ghani K, Garnier A, Coelho H, Transfiguracion J, Trudel P, Kamen A (2005), "Retroviral vector production using suspension-adapted 293GPG cells in a 3L acoustic filter-based perfusion bioreactor". Biotechnol Bioeng, 95(4):653-660.
  • Segura, MM, Garnier, A, Kamen, A (2006), "Purification and characterization of retrovirus vector particles by rate zonal ultracentrifugation". J Virol Methods, 133:82-91.
  • Segura MM, Kamen A, Garnier A (2005), "Downstream processing of oncoretroviral and lentiviral gene therapy vectors". Biotechnol Adv, 24(3):321-337.
  • Segura, MM, Kamen, A, Trudel, P, Garnier, A (2005), "A novel purification strategy for retrovirus gene therapy vectors using heparin affinity chromatography". Biotechnol Bioeng, 90(4):391-404.
  • Michaud, FT, Parent, V, Garnier, A, Duchesne, C (2005), "On the Proper Use of Statistical Design of Experiments". Exp Hematol, 33(11):1271-1272.
  • Cortin, V, Garnier, A, Pineault, N, Boyer, L, Lemieux, R, Proulx, C (2005), "Efficient In Vitro Megakaryocyte Maturation Using Cytokine Cocktails Optimized by Statistical Experimental Design". Exp Hematol, 33 (10): 1182-1191.
  • Cortin, V, Thibault, J, Jacob, D, Garnier, A (2004), "Recombinant High-titer adenovirus vector production in 293S cell perfusion culture." Biotechnol Progress, 20(3):858-63.
  • Jardon, M, Garnier, A (2003), "Effect of pH, PCO2, and Temperature on Recombinant Adenovirus Production". Biotechnol Prog, 19:202-208.
  • Boudreault, P, Tremblay, JP, Pépin, MF, Garnier, A. (2001), "Scale-Up of a Myoblast Cell Culture Process". J. Biotechnol., 91 (1):63-74.
  • El Zoeiby, A, Sanschagrin, F, Havugimana, PC, Garnier, A, Lévesque, RC (2001), "In vitro reconstruction of the biosynthetic pathway of peptidoglycan cytoplasmic precursor in Pseudomonas aeruginosa". FEMS Microbiol Lett, 201(2):229-235.
  • Pouliot, K, Thibault, J, Garnier, A, Acuna G (2000), "KLa evaluation during the course of a fermentation using data reconciliation techniques". Bioproc Eng, 23(6), 565-573.
  • Gilbert, PA, Garnier, A, Jacob, D, Kamen, A (2000), "On-line measurement of GFP fluorescence for the monitoring of recombinant adenovirus production". Biotechnol Letters, 22:561-567.
  • Côté, J, Garnier, A, Massie, B, Kamen, A (1998), “Serum-free production of recombinant proteins and adenoviral vectors by 293SF-3F6 cells”. Biotechnol Bioeng, 59(5):567-575.
  • Côté, J, Bourget, L, Garnier, A, Kamen, A (1997), “Study of adenovirus production in serum-free 293SF suspension culture by GFP-expression monitoring”. Biotechnol Progress, 13(6):709-714.
  • Jani, A, Lochmuller, H, Acsadi, G, Simoneau, M, Huard, J, Garnier, A, Karpati, G, and Massie, B (1997), "Generation, validation and large scale production of adenoviral recombinants with large size inserts such as a 6.3 kb human dystrophin cDNA". J Virol Meth, 64:111-124.
  • Ramsay, J, Rhazi, B, Garnier, A, Sapieha, S, Chavarie, C, Ramsay, B (1997), "Attachment of Vero cells to corona-treated, plastic surfaces". J Ferm Bioeng, 83:173-178.
  • Nadeau, I, Garnier, A, Côté, J, Massie, B, Chavarie, C, Kamen, A (1996), "Improvement of recombinant protein production with the human adenovirus/293S expression system using fed-batch strategies". Biotechnol Bioeng, 51(6): 613-623.
  • Garnier, A, Voyer, R, Tom, R, Perret, S, Jardin B, Kamen, A (1996), "Dissolved carbon dioxide accumulation in a large scale and high density production of TGF  receptor with baculovirus infected Sf-9 cells". Cytotechnology, 22: 53-63.
  • Kamen, A, Garnier, A, André, G, Archambault, J, Chavarie, C (1995), "Determination of mass transfer parameters in surface aerated bioreactors with bubble entrainment". Chem Eng J, 59: 187-193.
  • Garnier, A, Côté, J, Nadeau, I, Kamen, A, Massie, B (1994), "Scale-up of the adenovirus expression system for the production of recombinant protein in human 293S cells". Cytotechnology, 15: 145-155.
  • Chavarie, C, Karamanev, D, Godard, F, Garnier, A, André, G (1993): "Comparison of the Growth Kinetics between Three Different Strains of Thiobacillus Ferrooxidans on Ferrous Sulfate". Geomicrobiology J, 11: 57-63.
  • Garnier, A, Chavarie, C, André G, Klvana, D (1990), "The inverse fluidization airlift bioreactor, part I: Hydrodynamic studies". Chem Eng Comm, 98: 31-45.

  • Membre de l’Ordre des ingénieurs du Québec (OIQ)
  • Laboratoire d'optimisation des bioprocédés (LOB)
  • Réseau canadien de centres d'excellence sur les cellules souches (STEMCELLNET, RCE)
  • Réseau de thérapie cellulaire et tissulaire (ThéCell, FRSQ)
  • Association de thérapie génique du Québec (ATGQ)
  • Centre de recherche PROTEO (FRNTQ)
  • Laboratoire d’observation et d’optimisation des procédés (LOOP)
  • Société canadienne de génie chimique (CSChE)
  • American Institute of Chemical Engineering (AIChE)
  • American Chemical Society (ACS)

  • Ph.D. génie chimique, École Polytechnique de Montréal, 1992
  • M.Sc. génie chimique, École Polytechnique de Montréal, 1987
  • B. Ing., École Polytechnique de Montréal, 1985