Alain Garnier, ing., professeur titulaire
Pavillon Adrien-Pouliot, local 3550-B
T. 418 656-3106
F. 418 656-5993
alain.garnier@gch.ulaval.ca
Champs d'intérêts
  • Bioingénierie
  • Conception, développement, optimisation et mise à l’échelle de bioprocédés
  • Production de métabolites, protéines recombinantes, vecteurs viraux, vaccins et cellules thérapeutiques
  • Ingénierie des cultures cellulaires
  • Bioprocédés environnementaux, énergétiques, verts et durables

Enseignement

  • GCH-2100 Génie biochimique II
  • GCH-2105 Assainissement industriel
  • GCH-2005 Cinétique et catalyse
  • GCH-2006 Calcul des réacteurs chimiques
  • GCH-6000 Communication scientifique orale et écrite I
  • GCH-7003 Cinétique biochimique
  • GCH-8001 Communication scientifique orale et écrite II

Publications

  • Juneau PM, Garnier A, Duchesne C (2016), "Monitoring of adherent live cells morphology using the Undecimated Wavelet Transform Multivariate Image Analysis (UWT-MIA)". Biotechnol Bioeng, 114(1):141-153.
  • Tremblay-Bouliane, K., Tremblay, D., Moineau, S., Gaillet, B., Garnier, A. (2016), "Screening peptide-ore interactions with a phage display library for bioflotation application". CIM J, 7 (3).
  • Parent, V.A., Tremblay, J.P., Garnier, A. (2016), "Rational design of a serum-free culture medium for the growth of human myoblasts destined to cell therapy". Can. J. Chem. Eng., 94(10):1855–1862.
  • Brassard JA, Fekete N, Garnier A, Hoesli, CA (2016), " Hutchinson-Gilford progeria syndrome as a model for vascular aging". Biogerontol, 17(1):129-145.
  • Iliuta I, Garnier A, Iliuta MC (2015), "Enzymatic Fatty Acid Hydroxylation in a Liquid–Liquid Slug Flow Microreactor". Ind Eng Chem Res, 54:7787-7799.
  • Garnier A, Gaillet B (2015), "Analytical Solution of Luedeking-Piret Equation for a Batch Fermentation Obeying Monod Growth Kinetics". Biotechnol Bioeng, 112(12):2468-2474.
  • Juneau PM, Garnier A, Duchesne C (2015), "The Undecimated Wavelet Transform-Multivariate Image Analysis (UWT-MIA) for simultaneous extraction of spectral and spatial information”. Chemom. Int Lab Syst, 142:304-318
  • Hoesli CA, Juneau PM, Chevallier P, Duchesne C, Garnier A, Laroche G (2014), "A fluorophore-tagged RGD peptide to control endothelial cell adhesion to micropatterned surfaces". Biomaterials, 35(3), 879-890.
  • Segura MM, Mangion M, Gaillet B, Garnier A (2013), "New developments in lentiviral vector design, production and purification". Expert Opin Biol Ther, 13(7):987-1011.
  • Juneau PM, Garnier A, Duchesne C (2013), "Selection and tuning of a fast and simple phase-contrast microscopy image segmentation algorithm for measuring myoblast growth kinetics in an automated manner". Microsc Microanal, 19(4), 855-866.
  • Michaud FT, Havugimana PC, Duchesne C, Sanschagrin F, Bernier A, Lévesque RC, Garnier A (2012), "Cell culture tracking by multivariate analysis of raw LCMS data". Appl Biochem Biotechnol, 167(3):474-488.
  • Leysi-Derilou Y, Duchesne C, Garnier A, Pineault N (2012), "Single Cell Level Analysis of Megakaryocyte Growth and Development". Differentiation, 83:200-209.
  • Robert A, Cortin V, Garnier A, Pineault N (2012), "Megakaryocyte and platelet production from human cord blood stem cells". Methods Mol Biol, 788:219-247.
  • Pineault N, Cortin V, Boyer L, Garnier A, Robert A, Thérien C, Roy DC (2010), "Individual and synergistic cytokine effects controlling the expansion of cord blood CD34+ cells and megakaryocyte progenitors in culture". Cytotherapy, 13(4):467-480.
  • Gaillet B, Gilbert R, Broussau S, Pilotte A, Malenfant F, Mullick A, Garnier A, Massie B (2010), "High-level recombinant protein production in CHO cells using lentiviral vectors and the cumate gene-switch". Biotechnol Bioeng, 106(2):203-215.
  • Leysi-Derilou Y, Robert A, Duchesne C, Garnier A, Boyer L, Pineault N (2010), « Polyploid megakaryocytes can complete cytokinesis ». Cell Cycle, 9(13):2587-2597.  
  • Caron AW, Nicolas C, Gaillet B, Ba I, Pinard M, Garnier A, Massie B, Gilbert R (2009), "Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins". BMC Biotechnol, 9:42-52.
  • Michaud, FT, Garnier, A, Lemieux, L, Duchesne, C (2008), "Multivariate analysis of single quadrupole LCMS spectra for routine characterization and quantification of intact proteins". Proteomics Proteomics, 9 (3):746-756.
  • Segura MM, Garnier A, Di Falco MR, Whissell G, Meneses-Acosta A, Arcand N, Kamen A (2007), " Identification of host proteins associated with  retroviral vector particles by proteomic analysis of highly purified vector preparations". J Virol, 82:1107-1117.
  • Segura MM, Garnier A, Durocher Y, Coelho H, Kamen A (2007), "Production of Lentiviral Vectors by Large-Scale Transient Transfection of Suspension Cultures and Affinity Chromatography Purification". Biotechnol Bioeng, 98 (4), 789-799.
  • Gilbert PA, Kamen A, Bernier A, Garnier A (2007), "A simple macroscopic model for the diffusion and adsorption kinetics of r-adenovirus". Biotechnol Bioeng., 98:239:251.
  • Gaillet B, Gilbert R, Amziani R, Guilbault C, Gadoury C, Mullick A, Garnier A, Massie, B (2007), "High-level recombinant protein production in CHO cells using an adenoviral vector and the cumate gene-switch". Biotechnol Prog, 23(1):200-209.
  • Paradis-Bleau C, Cloutier I, Lemieux L, Sanschagrin F, Laroche J, Auger M, Garnier A, Levesque R (2007), "Peptidoglycan lytic activity of the Pseudomonas aeruginosa phage PhiKZ gp144 lytic transglycosylase". FEMS Microbiol Lett, 266:201-209.
  • Segura MM, Kamen A, Lavoie M-C, Garnier A (2007), "Exploiting heparin-binding properties of MoMLV-based retroviral vectors for affinity chromatography". J Chrom B, 846:124-131.
  • Ghani K, Garnier A, Coelho H, Transfiguracion J, Trudel P, Kamen A (2005), "Retroviral vector production using suspension-adapted 293GPG cells in a 3L acoustic filter-based perfusion bioreactor". Biotechnol Bioeng, 95(4):653-660.
  • Segura, MM, Garnier, A, Kamen, A (2006), "Purification and characterization of retrovirus vector particles by rate zonal ultracentrifugation". J Virol Methods, 133:82-91.
  • Segura MM, Kamen A, Garnier A (2005), "Downstream processing of oncoretroviral and lentiviral gene therapy vectors". Biotechnol Adv, 24(3):321-337.
  • Segura, MM, Kamen, A, Trudel, P, Garnier, A (2005), "A novel purification strategy for retrovirus gene therapy vectors using heparin affinity chromatography". Biotechnol Bioeng, 90(4):391-404.
  • Michaud, FT, Parent, V, Garnier, A, Duchesne, C (2005), "On the Proper Use of Statistical Design of Experiments". Exp Hematol, 33(11):1271-1272.
  • Cortin, V, Garnier, A, Pineault, N, Boyer, L, Lemieux, R, Proulx, C (2005), "Efficient In Vitro Megakaryocyte Maturation Using Cytokine Cocktails Optimized by Statistical Experimental Design". Exp Hematol, 33 (10): 1182-1191.
  • Cortin, V, Thibault, J, Jacob, D, Garnier, A (2004), "Recombinant High-titer adenovirus vector production in 293S cell perfusion culture." Biotechnol Progress, 20(3):858-63.
  • Jardon, M, Garnier, A (2003), "Effect of pH, PCO2, and Temperature on Recombinant Adenovirus Production". Biotechnol Prog, 19:202-208.

Affiliations

  • Membre de l’Ordre des ingénieurs du Québec (OIQ)
  • Laboratoire d'optimisation des bioprocédés (LOB)
  • Réseau canadien de centres d'excellence sur les cellules souches (STEMCELLNET, RCE)
  • Réseau de thérapie cellulaire et tissulaire (ThéCell, FRSQ)
  • Association de thérapie génique du Québec (ATGQ)
  • Centre de recherche PROTEO (FRNTQ)
  • Laboratoire d’observation et d’optimisation des procédés (LOOP)
  • Société canadienne de génie chimique (CSChE)
  • American Institute of Chemical Engineering (AIChE)
  • American Chemical Society (ACS)

 

Formation et expériences professionnelles

  • B. Ing., École Polytechnique de Montréal, 1985
  • M.Sc. génie chimique, École Polytechnique de Montréal, 1987
  • Ph.D. génie chimique, École Polytechnique de Montréal, 1992
 
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